Character and laxative activity of polysaccharides isolated from Dendrobium officinale

2018-11-23 本站

题目：Character and laxative activity of polysaccharides isolated from Dendrobium officinale

期刊：Journal of Functional Foods

作者：DandanLuo1，CaoQu2，GuoshengLin1，ZhenbiaoZhang1，JianhuiXie3，HanbinChen4，JialiLiang1，CailanLi1，HongfengWang5,6，ZirenSu1,7，

单位：1.School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China；2.Guangdong Province Forestry Science and Technology Extension Station, Guangzhou, Guangdong 510173, PR China；3.Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510000, PR China；4.The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China；5.Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangzhou 510000, PR China；6.Guangdong Academy of Forestry, Guangzhou, Guangdong 510520, PR China；7.Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China

摘要： Dendrobium officinale was separated into total polysaccharides (DOP) and supernatant (DOS) to investigate the laxative ingredients and mechanism in normal and constipated mice induced by diphenoxylate or water fasting. The molecular weight and components of DOP were determined by HPGPC, HPLC and LC-MS. DOP was mainly composed of d-mannose (59.19 mg/g) and d-glucose (830.98 mg/g), with a molecular weight of 8404 Da. DOP (29, 57, 114 mg/kg, i.g.) exhibited an appreciable laxative effect in normal mice (p < 0.05), while DOS (35, 70, 140 mg/kg, i.g.) did not. DOP significantly enhanced the GI transit ratio; recovered the faecal output characteristics; obviously increased the levels of Gas, MTL, AChE and SP; and decreased the levels of SS in constipated mice (all p < 0.05). The current study suggested that DOP is the laxative component of D. officinale and can be used as a promising dietary therapy for constipation in daily life.

关键词：Dendrobium officinale，Polysaccharides，Laxative，Constipation，Diet therapy